Activity analysis in the CLAMS monitored by infrared beam breaks. All data were analyzed with the t test with Welch's correction and the InSTAT3 software (GraphPad). A, Total activity (walking and grooming) in the deletion mutant and control females with a significant decrease in deletion mutants, during both dark and light phases (P < 0.0001). B, Ambulatory activity in the x-axis (walking) is also reduced in mutant females (P < 0.0001). C, There is a reduction in z-axis activity (rearing or jumping) in mutant females (P < 0.0001). D, Slight increases in x-axis activity (ambulation) is shown in mutant males (P < 0.0001). E, Graphs of the increased z-axis activity seen in the mutant males (P < 0.0001). F, Illustration of the sleep analysis indicating that mutant males spend a lower percent of time sleeping than controls (P < 0.001). Closed star, Significantly greater than control values; open star, significantly different from values in light phase or, in the case of panel F, significantly different from males.