Fig. 5.

The TRE in X. tropicalis IYD promoter mediates activation by liganded TR. A, Schematic diagrams show the reporter constructs of the wild-type and mutant of X. tropicalis IYD promoter. B, Mutating the TRE eliminates the induction of the promoters by T₃. Wild-type or mutant (mTRE) promoter construct was coinjected with the control Renilla luciferase construct phRG-tk into the nuclei of the oocytes with or without prior cytoplasmic injection of mRNA for X. tropicalis TRα and RXRβ. The oocytes were incubated at 18 C overnight in the presence or absence of 100 nm T₃ and then used for dual-luciferase assays. The relative activities of the firefly luciferase to Renilla luciferase were plotted. Error bars indicate sem (n = 3).