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Imaging of neurons expressing either actin-CFP or actin-YFP alone to estimate the bleed-through between two channels under different excitation wave length. PV neurons in cortical slice culture were transfected with LSL-actin-CFP or LSL-actin-YFP at EP14. The labeled neuron was imaged with two-photon microscopy at EP18. Images were taken under different wavelength excitation indicated in the figure.
