Figure 5.

Distance determinations from the LOPTC spin to spins on SBL1. (A) Five single R₁ SBL1 mutants (200 μM) with 0.8 equivalents of LOPTC, in TRICINE (0.1 M, pH 8.4 at 22°C), sucrose 30% (w/v), were examined by DEER, at 60 K. The maxima in the P(r) distributions are given in the footnote of Table 1. (B) Similar experiments measuring the distance between F782R₁ and 8DSA, as the substrate analog, yielded a P(r) maximum at ∼30 Å. The 8-doxylstearic acid (8DSA) was added from an ethanol solution giving 1% ethanol in the final sample.