Skip to main content
. 2012 Nov 21;103(10):2115–2124. doi: 10.1016/j.bpj.2012.10.008

Figure 1.

Figure 1

Representation of the modifications to the FhuA protein nanopore. (A) Diagram showing side (upper) and top (lower) views of the crystal structure of the wild-type (FhuA) and modified (FhuA ΔC/Δ4L) proteins with the extracellular loops—L3, L4, L5, and L11—labeled. (B) Surface representation of the engineered FhuA ΔC/Δ4L protein nanopore and its expected orientation in a synthetic planar lipid bilayer. The cartoon was made using the Protein Data Bank structure 1BY5 of the native FhuA protein (7). On the right side, the graph shows the expected internal diameter of the FhuA ΔC/Δ4L protein nanopore along the longitudinal axis, as calculated using the HOLE program (61).