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Voltage dependence of the conductance ratio of the FhuA ΔC/Δ4L nanopore, which was induced by the presence of PEGs in the bilayer chambers. Indicated are the currents measured at different voltages for the FhuA ΔC/Δ4L nanopore in 300-Da PEG cis and 12000-Da PEG trans (circles), 12000-Da cis and 300-Da PEG trans (triangles), and 300-Da PEG symmetric (squares). Solutions were 15% (w/w) PEG, in 1 M KCl and 10 mM potassium phosphate, pH 7.4.
