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Backbone atoms (residues 62–66) of two opposite subunits of the SF of NaK-CNG from the energy minima I–XI. The last frames of the umbrella-sampling trajectories with harmonic restraints closer to the energy minima are superimposed. Spheres are used to highlight the positions of the carbonyl oxygen atoms. Colors range from red (trajectory closer to minimum I) to blue (trajectory closer to minimum XI).
