Fig. 2.

Effect of GC-1 on bile acid synthesis and fecal excretion. Male LDLR^−/− mice were administered GC-1 (0.8 mg/kg diet) in a Western diet containing 0.2% cholesterol or control diet lacking GC-1. A, mRNA concentrations were measured by real-time quantitative PCR using total mRNA prepared from either liver (Cyp7a1) or ileum (I-BABP and ASBT) sections of mice treated with GC-1 for 10 d (n = 5 per group). B, Serum C4 levels were measured via mass spectrometry and normalized relative to serum cholesterol levels. C and D, Feces were collected from d 5 to d 8 of treatment and analyzed for bile acids and neutral sterols (n = 4–5 per group). *, P < 0.05; **P < 0.01; ***, P < 0.001.