Table 2.

Seminiferous tubules from rats displaying a loss of spermatid polarity at different stages of the seminiferous epithelial cycle after knockdown of different Scribble components by RNAi in vivo^a

Treatment	Seminiferous tubules (%) displaying loss of spermatid polarityb (mean ± sd)
	I–IV	VII–VIII	IX–XIV
Control RNAi	2 ± 2	9.33 ± 3.06	3.25 ± 1.09
Scribble RNAic	3.33 ± 1.15	10.67 ± 2.31 (ns)	2.67 ± 3.06
Lgl2 RNAi	3.15 ± 1.99	8.7 ± 0.61 (ns)	4 ± 2
SLD RNAic	3.33 ± 3.06	22.67 ± 4.16**	4 ± 2

ns, Not significantly different;

^**, P < 0.01 when compared with control RNAi group.

^aTestes from three animals in each treatment group were analyzed, with at least approximately 50–60 tubules randomly selected from the testes.

^bTubules that contained more than 10 spermatids with loss of polarity were scored as damaged tubules.

^cIn Scribble RNAi or SLD RNAi testes, only tubules with reduced Scribble expression as the result of Scribble silencing were selected for analysis.