Fig. 8.

The presence of extracellular Ca²⁺ is essential for a sustained Kp-10-induced Ca²⁺ response. CHO-KISS1R cells were treated with 100 nm Kp-10 in the presence or absence of extracellular calcium in the medium. The mobilization of intracellular Ca²⁺ was measured as relative fluorescence units (RFU) every 15 sec for 7.5 min. A single representative experiment is shown, which was repeated three times with similar results. The values shown represent the ratio of the difference between Kp-10-stimulated and basal values divided by the basal value at each time point.