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. 2012 Nov 2;153(12):5993–6002. doi: 10.1210/en.2012-1531

Fig. 6.

Fig. 6.

A, Coimmunoprecipitation assay showing the detection of GR in the nuclear protein complex precipitated by p300 antibody upon stimulation of human amnion fibroblasts with cortisol (F; 1 μm). Preimmune IgG served as negative control. B, Diagram illustrating the positions of putative GREs and primer sets used for the ChIP assay. C and D, Top panels are the representative gel images showing the abundance of PCR products, and bottom panels are the mean data of qRT-PCR showing the enrichment of p300, GR to H6PD promoter upon cortisol (F; 1 μm) stimulation of human amnion fibroblasts. Preimmune IgG served as negative control (n = 3–4). *, P < 0.05 vs. control (CTR). TSS, Transcription start site; PP1, proximal primer set 1; PP2, proximal primer set 2; DP, distal primer set.