Table 1.
Primer sequences used in qRT-PCR
| Genes | Primer sequence (5′–3′) | Accession no. | Product size (bp) |
|---|---|---|---|
| Primers for mRNA measurement | |||
| H6PD | Forward, CTGCAGCACGTCCGGATCCC | NM_004285.3 | 298 |
| Reverse, TGGCGCGGTTGATGAGAGGC | |||
| P300 | Forward, AGGCGTCGAGGACCTGGTGT | NM_001429.3 | 197 |
| Reverse, TGGCCATTGTAACCAGAAATGGGC3 | |||
| β-Actin | Forward, TCCTCCTGAGCGC AAGTACTCT | NM_001101.3 | 297 |
| Reverse, GCTCAGTAACAGTCCGCCTAGAA | |||
| Primers for ChIP assay | |||
| Name | Primer sequence | Location | Product size (bp) |
| PP1 | Forward, GACGCGGCAGGACAGCTCTA | −244, 146 | 390 |
| Reverse, GCCCGTCACTCCGACAAGTGC | |||
| PP2 | Forward, GCGTGTGCGCTCCGACTTCT | −445, −269 | 176 |
| Reverse, GGCCGAGGGACGTGGTTGTG | |||
| DP | Forward, TTCTCCTGAGATCCCGCTCA | −8993, −8825 | 169 |
| Reverse, TGCACGTGGTGTAAGCTAGG |
PP1, Proximal primer set 1; PP2, proximal primer set 2; DP, distal primer set.