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. 2012 Dec 3;2:916. doi: 10.1038/srep00916

Figure 1. Identification of the region responsible for PMA-induced promoter activity in the H1R gene.

Figure 1

Serial deletion MTs were constructed as described in Supplementary methods using the primary construct p2029 (2.1-kb DNA fragment from the upstream regulatory region of the H1R gene). HeLa cells were cotransfected with these MT plasmids and pRL-MPK. After 5 h, cells were serum starved for 24 h and then treated with 100 nM of PMA for 10 h. Luciferase activity was determined using the Dual-Luciferase Reporter Assay System. Data are presented as mean ± S.E.M. (n = 9). *, p < 0.01 (t-test).