Figure 1.

Time-course experiments of the induction and degradation of mtCA. A, Northern analysis of the relative amounts of the mtCA mRNA. High-CO₂-grown cells were transferred to low-CO₂ conditions at time 0. After 24 h, the culture was transferred back to high-CO₂ conditions. The triangle represents cells left on low CO₂ for 1 week. All lanes on the northern blot contained 5 μg of total RNA. B, Western blot of the induction of mtCA. Numbers represent hours after transfer to low CO₂. All lanes contained 0.5 μg of protein. C, Western blot of the degradation of mtCA. Numbers represent hours after transfer from low CO₂ to high CO₂. All lanes contained 0.5 μg of protein.