Figure 6 .

Insertion of multi-spanning carrier proteins into the inner membrane. Newly synthesized multi-spanning carrier proteins, complexed with cytoplasmic Hsp70, are recognized by the Tom70 receptor subunit of the TOM complex. ATP-dependent release from cytoplasmic Hsp70 leads to translocation through the TOM complex in a looped configuration and binding to the Tim9-Tim10 IMS chaperone complex. The multi-spanning proteins are delivered to the TIM22 insertase complex in the inner membrane, released from Tim9-Tim10, and inserted into the inner membrane by reactions that depend upon the Δψ potential across the inner membrane.