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. 2008 Aug 13;28(33):8246–8256. doi: 10.1523/JNEUROSCI.1941-08.2008

Figure 4.

Figure 4.

IKKβ and p65 phosphorylation in SCG and nodose neurons. A, B, Representative Western blots comparing the levels of phospho-IKK and total IKK (A) and phospho-S536-p65 and total p65 (B) in the same quantity of total protein extracted from P0 SCG and nodose neurons 24 h after plating. C, Quantification of the relative levels of these proteins from densitometric scans of three separate experiments of each type are plotted. D, Photomicrograph showing immunostainings of phospho-S536-p65 in cultured P0 nodose and SCG neurons 24 h after transfection with a YFP plasmid together with an IKKβ plasmid. DAPI nuclear staining, YFP labeling, and phospho-S536-p65 immunostaining in the same fields are shown from left to right. The bar chart shows the relative intensity of phospho-S536-p65 immunostaining in IKKβ-overexpressing nodose and SCG neurons (black bars) normalized to the level in the respective control-transfected nodose and SCG neurons (pcDNA3.1 plasmid, white bars). E, Photomicrograph of immunostainings for total p65 in cultured P0 nodose and SCG neurons 24 h after transfection with a YFP plasmid together with a p65 plasmid. DAPI staining, YFP labeling, and p65 immunostaining in the same fields are shown from left to right. The bar chart shows the relative intensity of p65 immunostaining in p65-overexpressing nodose and SCG neurons (black bars) normalized to the level in the respective control-transfected nodose and SCG neurons (pcDNA3.1 plasmid, white bars). F, Photomicrograph of immunostainings for phospho-S536-p65 in cultured P0 nodose and SCG neurons 24 h after transfection with a YFP plasmid together with a p65 plasmid. DAPI staining, YFP labeling, and phospho-S536-p65 immunostaining in the same fields are shown from left to right. The bar chart shows the relative intensity of phospho-S536-p65 immunostaining in p65-overexpressing nodose and SCG neurons (black bars) normalized to the level in the respective control-transfected nodose and SCG neurons (white bars). Means and SEs of measurements from >150 neurons for each condition are shown in D–F. Statistically significant difference from control-transfected neurons is indicated (**p < 0.001).