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. Author manuscript; available in PMC: 2012 Dec 3.

Published in final edited form as: Mod Pathol. 2011 Nov 18;25(2):246–259. doi: 10.1038/modpathol.2011.164

Co-expression of CD39 and CCR7 in conventional T regulatory (T_regs) in healthy donors and chronic lymphocytic leukemia and autologous T_reg-mediated suppression of polyclonal T-cell responses from healthy donors and chronic lymphocytic leukemia. Conventional T_regs were identified after staining as described earlier. Expression of the activation markers CD39 and CCR7 were measured. (a) Representative dot plot of CD39 and CCR7 co-expression pattern on conventional T_regs in a healthy donor (left panel) and chronic lymphocytic leukemia (right panel). (b) Histograms of the mean percentages (±s.e.m.) of T_regs expressing these markers in healthy donors (black) and chronic lymphocytic leukemia (gray). (c) T_regs were isolated using flow cytometric sorting as described earlier. Expression of CD25 and CD4 were used to identify T_reg cells in both healthy donors and in chronic lymphocytic leukemia. A representative dot plot of CD39 and CCR7 expression pattern in a healthy donor (upper panel) and chronic lymphocytic leukemia (lower panel). (d) Suppressive capacity of T_regs toward responder cells (T_effec) was expressed as relative inhibition of the percentage of CSFE-low cells.

Co-expression of CD39 and CCR7 in conventional T regulatory (T_regs) in healthy donors and chronic lymphocytic leukemia and autologous T_reg-mediated suppression of polyclonal T-cell responses from healthy donors and chronic lymphocytic leukemia. Conventional T_regs were identified after staining as described earlier. Expression of the activation markers CD39 and CCR7 were measured. (a) Representative dot plot of CD39 and CCR7 co-expression pattern on conventional T_regs in a healthy donor (left panel) and chronic lymphocytic leukemia (right panel). (b) Histograms of the mean percentages (±s.e.m.) of T_regs expressing these markers in healthy donors (black) and chronic lymphocytic leukemia (gray). (c) T_regs were isolated using flow cytometric sorting as described earlier. Expression of CD25 and CD4 were used to identify T_reg cells in both healthy donors and in chronic lymphocytic leukemia. A representative dot plot of CD39 and CCR7 expression pattern in a healthy donor (upper panel) and chronic lymphocytic leukemia (lower panel). (d) Suppressive capacity of T_regs toward responder cells (T_effec) was expressed as relative inhibition of the percentage of CSFE-low cells.