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. Author manuscript; available in PMC: 2012 Dec 3.
Published in final edited form as: Mod Pathol. 2011 Nov 18;25(2):246–259. doi: 10.1038/modpathol.2011.164

Figure 5.

Figure 5

CD4+CD25−Tregs and CD8 Tregs, their memory vs naïve subtypes, and their activation status in healthy donors and chronic lymphocytic leukemia. Cells were stained with antibodies and analysis was performed to identify CD25−Tregs and CD8 Tregs as described earlier. (a) The graphs display the percentages and the mean percentage values (±s.e.m.) of CD25−Tregs in healthy donors (black) and chronic lymphocytic leukemia (gray). (b) The graphs show the percentages and the mean percentage values (±s.e.m.) of central memory (CM) CD25−Tregs (identified as CD27+CCR7+ cells among CD45RA−CD25−Tregs), of effector memory (EM) CD25−Tregs (identified as CD27+ CCR7− cells among CD45RA−CD25−Tregs), of effector CD25−Tregs (identified as CD27−CCR7− cells among CD45RA−CD25−Tregs), and naïve CD25−Tregs (identified as cells among CD25−Tregs). (c) The graphs display the mean percentage values (±s.e.m.) of CD25−Tregs expressing activation markers CD38, CD39, CD103, CD127, and HLA-DR in healthy donors (black) and chronic lymphocytic leukemia (gray). (d) The graphs represent the percentages and the lines indicate the mean percentage value (±s.e.m.) of CD8 Tregs in healthy donors (black) and chronic lymphocytic leukemia (gray). (e) The graphs show the percentages and the lines indicate the mean percentage value (±s.e.m.) of CM CD8 Tregs (identified as CD27+CCR7+ cells among CD45RA−CD8 Tregs), of effector memory (EM) CD8 Tregs (identified as CD27+CCR7− cells among CD45RA−CD8 Tregs), of effector CD8 Tregs (identified as CD27−CCR7− cells among CD45RA−CD8 Tregs), and naïve CD8 Tregs (identified as CD45RA+ cells among CD8 Tregs).