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Ex vivo hyporesponsiveness of NK cells from SHIP−/− mice. Intracellular IFN-γ (A) and CD107α (B) in NK cell populations (NK1.1+CD3− in the lymphocyte gate) from total splenocytes incubated with a mix of IL-12 and IL-18 or PMA and ionomycin (P/I) for 6 hours. Data are pooled from at least 3 independent experiments. Error bars indicate SEM.
