Table 1.
Expression of maturation markers on NK cells from SHIP-1 deficient mice and littermate controls*
| BM | SHIP+/+ | SHIP+/− | SHIP−/− | Spleen | SHIP+/+ | SHIP+/− | SHIP−/− |
|---|---|---|---|---|---|---|---|
| cKit | 5.3 ± 0.5 | 5.8 ± 1.5 | 6.5 ± 3.4 | cKit | 6.5 ± 1.0 | 6.6 ± 0.4 | 21.4 ± 6.9 |
| CD51 | 5.5 ± 0.4 | 8.2 ± 2.0 | 8.5 ± 0.7 | CD51 | 3.7 ± 0.1 | 9.3 ± 1.9 | 42.2 ± 5.9 |
| CD94 | 45.1 ± 0.4 | 48.3 ± 4.1 | 56.2 ± 8.4 | CD94 | 45 ± 1.3 | 56.0 ± 0.9 | 58.3 ± 7.3 |
| Ly49s* | 69.27 ± 0.9 | 69.1 ± 5.5 | 67.0 ± 13.21 | Ly49s* | 69.7 ± 0.4 | 64.5 ± 2.0 | 35.5 ± 17.8 |
| CD43 | 97.4 ± 2.1 | 96.2 ± 1.6 | 97.4 ± 2.6 | CD43 | 99.3 ± 0.1 | 98.4 ± 0.5 | 92.8 ± 3.5 |
| CD27 | 70.0 ± 5.3 | 76.7 ± 3.2 | 79.3 ± 4.4 | CD27 | 64.2 ± 2.1 | 69.4 ± 2.8 | 85.1 ± 2.4 |
| DX5 | 94.9 ± 0.4 | 94.4 ± 1.1 | 84.9 ± 1.5 | DX5 | 92.2 ± 0.3 | 91.5 ± 0.9 | 68.5 ± 11.7 |
| CD11b | 61.6 ± 2.8 | 47.7 ± 2.1 | 12.2 ± 2.2 | CD11b | 84.3 ± 1.7 | 73.2 ± 0.7 | 21.7 ± 8.4 |
| KLRG1 | 12.6 ± 2.4 | 10.1 ± 1.6 | 4.6 ± 0.5 | KLRG1 | 21.5 ± 2.4 | 21.8 ± 5.3 | 10.8 ± 3.3 |
Results are expressed as mean frequency ± SD of 3 mice per genotype and are representative of at least five independent experiments. Values in bold represent P < .05 versus controls (SHIP+/+ and SHIP+/−). % marker-positive NK cells.
*
Results from a pool of antibodies specific for Ly49C/I/F/H and Ly49G2.