Table II.
Effect of re-aeration on levels of GSH and GSSG in roots of hypoxically (H) or anoxically (A) pretreated wheat seedlings compared with aerated control (C)
| Treatment | GSSG + GSH | GSH | GSSG | GSH/GSSG | GSH |
|---|---|---|---|---|---|
| nmol g−1 fresh wt | ratio | % | |||
| C | 576 | 488 (68) | 88 (18) | 5.5 | 84.7 |
| H | 657 | 579 (35)* | 78 (10) | 7.4 | 88.1 |
| H + 20 min C | 668 | 564 (24) | 104 (18)* | 5.4 | 84.5 |
| H + 2 min h C | 726 | 653 (83) | 74 (15) | 8.8 | 89.9 |
| H + 16 h C | 956 | 910 (164)* | 46 (7) | 19.8 | 95.2 |
| C | 595 | 509 (47) | 85 (14) | 5.9 | 85.7 |
| C + 2 d A | 455 | 400 (91) | 55 (12)* | 7.3 | 88.0 |
| C + 2 d A + 20 min C | 441 | 369 (36) | 72 (19) | 5.1 | 83.7 |
| C + 2 d A + 2 h C | 478 | 408 (10) | 69 (14) | 5.9 | 85.5 |
| C + 2 d A 16 h C | 594 | 533 (85) | 62 (9) | 8.6 | 89.7 |
| C | 521 | 443 (4) | 77 (20) | 5.8 | 85.2 |
| C + 4 d A | 333 | 304 (8)* | 29 (11)* | 10.5 | 91.2 |
| C + 4 d A + 20 min C | 373 | 326 (40) | 47 (6)* | 6.9 | 87.3 |
| C + 4 d A + 20 min C | 392 | 354 (47) | 37 (9) | 9.6 | 90.5 |
| C + 4 d A + 16 h C | 518 | 485 (34)* | 33 (3) | 14.6 | 93.6 |
Each value represents the mean of at least five independent experiments. sd values are shown in parentheses. Asterisks indicate significant differences at the 5% level between values obtained under control and hypoxia or anoxia, and following re-aeration after hypoxia or anoxia. For statistical analysis, see Table I.