Hrp1 and Hrp3 together are critical for the generation of TSS-aligned genic nucleosome arrays. Overlay of TSS-aligned nucleosome occupancy profiles for 4013 genes in wt (Hu303, average of five biological replicates) and (A) hrp1Δ (Hu2239, average of two biological replicates), (B) hrp3Δ (Hu0575/EJY321, average of two biological replicates) and (C) hrp1Δ hrp3Δ (Hu2303, average two biological replicates) at 30°C. (D–F) Spectral analysis as in (Lantermann et al, 2010) for the data in panels (A–C), respectively. 6.2 or 6.5 nucleosomes per 1000, bp translate into a NRL of 161 or 154 bp in hrp1Δ or wt, respectively.