Skip to main content
. 2012 Oct 26;31(23):4453–4465. doi: 10.1038/emboj.2012.295

Figure 6.

Figure 6

Thymic HDAC7-ΔP expression causes multi-organ visceral autoimmunity. (A) Immunofluorescence staining of pancreatic sections from Rag1-deficient mice with sera from wild-type littermate control (top left) and HDAC7-ΔP TG (top right) sera. Bottom left shows reactivity of rag1 KO sera, bottom right signal from incubation with secondary antibody only. (B) Representative western blots showing reactivity of wild-type littermate control (top) and HDAC7-ΔP TG (bottom) serum against a panel of whole tissue extracts from Rag1-deficient animals. (C) Representative haematoxylin and eosin stained sections of wild-type control (left) and HDAC7-ΔP TG (right) stomach (top) and liver (bottom), showing immune infiltrates and local tissue damage (yellow arrows). (D) Percent survival of cohorts of six Rag1 KO littermate control (blue line) and six Rag1 KO/HDAC7-ΔP TG mice (red line) from 12 to 26 weeks of age. (E) Percent survival from 0 to 12 weeks post transfer of four cohorts of eight Rag1 KO mice transferred with wild-type B cells and T cells from WT and HDAC7-ΔP TG mice as indicated in legend. (F) Representative haematoxylin and eosin stained sections of pancreas from two mice engrafted with wild-type T and B cells (left) and two HDAC7-ΔP T+wild-type B cell-engrafted mice (right), showing destructive infiltrates in the mice receiving HDAC7-ΔP T cells (yellow arrows).