Inhibition by IDP (A) and glucosamine (B) of the
oxidative burst induced by hypoosmotic stress in tobacco cell
suspensions. A, Aliquots of cell suspension were equilibrated for
3 h in isoosmotic medium before transfer at 0 time to hypoosmotic
(Hypo), isoosmotic (Iso), hypoosmotic containing 20 μm
IDP (Hypo-IDP), or isoosmotic containing 20 μm IDP
(Iso-IDP) media. The inhibitor was also added during the last 15 min of
the equilibration time at the concentration used after transfer. B,
Aliquots of cell suspension were equilibrated for 2 h in buffered
culture medium and for an additional 1 h in the same medium
containing 10 mm glucosamine, before transfer in
hypoosmotic medium containing glucosamine (Hypo-Gl) or isoosmotic
medium containing glucosamine (Iso-Gl). Corresponding controls were
treated similarly, except for the replacement of glucosamine by 30
mm Man during the last 1 h of equilibration and after
transfer in hypoosmotic (Hypo) or isoosmotic (Iso) conditions.
Means ± se of two independent experiments are
reported in each case. FW, Fresh weight.