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. 1998 Feb;116(2):659–669. doi: 10.1104/pp.116.2.659

Figure 2.

Figure 2

Inhibition by IDP (A) and glucosamine (B) of the oxidative burst induced by hypoosmotic stress in tobacco cell suspensions. A, Aliquots of cell suspension were equilibrated for 3 h in isoosmotic medium before transfer at 0 time to hypoosmotic (Hypo), isoosmotic (Iso), hypoosmotic containing 20 μm IDP (Hypo-IDP), or isoosmotic containing 20 μm IDP (Iso-IDP) media. The inhibitor was also added during the last 15 min of the equilibration time at the concentration used after transfer. B, Aliquots of cell suspension were equilibrated for 2 h in buffered culture medium and for an additional 1 h in the same medium containing 10 mm glucosamine, before transfer in hypoosmotic medium containing glucosamine (Hypo-Gl) or isoosmotic medium containing glucosamine (Iso-Gl). Corresponding controls were treated similarly, except for the replacement of glucosamine by 30 mm Man during the last 1 h of equilibration and after transfer in hypoosmotic (Hypo) or isoosmotic (Iso) conditions. Means ± se of two independent experiments are reported in each case. FW, Fresh weight.