Involvement of protein phosphorylation in the
activation of oxidative burst by hypoosmotic stress. Aliquots of cell
suspension were equilibrated for 3 h in isoosmotic medium before
transfer at 0 time to hypoosmotic (Hypo), isoosmotic (Iso), or
hypoosmotic medium containing 0.5 μm staurosporine (A,
Hypo-Stau), 500 μm DMAP (B, Hypo-DMAP), 500
μm apigenin (C, Hypo-Api), or isoosmotic medium
containing the same inhibitor concentrations (A, Iso-Stau; B, Iso-DMAP;
and C, Iso-Api). One representative experiment of three independent
experiments is illustrated in each case. FW, Fresh weight.