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Western blot analysis of normal lung epithelial cells (CRL-9482) and lung cancer cells (NCI-H1299 and A549) stably transduced with MLV-backbone (BB) or MLV-HA-RASSF1C (1C) treated with 1 μg/ml doxycycline for 48 hrs. The anti-HA tag antibody detected a HA-RASSF1C fusion protein of the expected size.
