Figure 1.

Purification of the 64-kD protein. Protein fractions were analyzed in a 7% (w/v) acrylamide gel and stained with Coomassie blue. Lanes 1 and 2, Barley thylakoid membranes (20 μg of chlorophyll) before and after extraction with STT buffer; lane 3, STT-extracted proteins (40 μg protein); lane 4, purified 64-kD protein (approximately 2 μg of protein); and lane 5, CF₁ complexes purified by Suc-gradient centrifugation (20 μg of protein). Molecular mass markers (in kD) are indicated at left.