Figure 2.

Effects of long-term growth at elevated CO₂ on Rubisco content and rbcS and rbcL transcript abundance. Absolute amounts of leaf Rubisco were 33.7 and 22.3 nmol g⁻¹ fresh weight for ambient- and high-CO₂-grown plants, respectively. One microgram of total RNA per lane was used for northern-blot analysis. rRNA was used as an internal control to ensure equal loading of the lanes; 25S rRNA is shown stained with methylene blue. Relative hybridization signals quantified by phosphor imaging were expressed as a percentage of the values obtained from the ambient control. Values are means ± sd from three filters each, with RNA samples extracted from two replicate experiments. Leaves were collected at midday.