Figure 9.

In vivo treatment with rosiglitazone reduces macrophage CD36 protein expression in ob/ob LDLR^–/– mice while increasing its expression in LDLR^–/– mice. (A) Western analysis of macrophage CD36 and SR-A protein expression from mice with or without rosiglitazone (RSG) treatment for 3 weeks as described in Methods. (B) For comparison, the expression of CD36 protein in adipose tissue of mice described in A was determined by Western analysis. CD36 protein is upregulated by PPAR-γ activator in adipose tissue of both ob/ob LDLR^–/– and LDLR^–/– mice. All mice treated with RSG showed a similar increase in CD36 protein expression in adipose tissue, and results from one mouse of each strain were shown here. Macrophage experiments were performed with pooled cells isolated from three mice of each strain. One experiment representative of two independent experiments is shown.