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2D PAGE gel from barley roots grown under Fe-sufficient (+Fe) and Fe-deficient (−Fe) conditions. Each gel was loaded with 200 μg of root proteins. Several spots of proteins that increased under Fe deficiency are indicated with arrowheads. C, Adenine phosphoribosyltransferase; Y, IDS3; and W, FDH. Other spots are unknown (Table I). Mr is reported in thousands.
