Figure 5.  .

Double-labeling for VEGFR2 (A–D), CD31 (E–H), and CD34 (I–L) with Hb-ε and DAPI in BIs of the vitreous at 6 WG. VEGFR2 and Hb-ε were coexpressed in free erythroblasts (arrowhead in A–D) and in some cells within BI (arrow in A–D). All cells of the BI were VEGFR2⁺. In contrast, CD31/Hb-ε was coexpressed in select cells of BI (arrow in E–H) while free Hb-ε⁺ erythroblasts were only weakly immunoreactive for CD31 (arrowhead in E–H). CD34/Hb-ε was coexpressed in some scattered cells in BIs (arrows in I–L), while free Hb-ε⁺ erythroblasts were CD34⁻ (not shown). Scale bar in A, E, I = 10 μm.