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. 2012 Dec 3;7(12):e50954. doi: 10.1371/journal.pone.0050954

Figure 1. In vitro study of the affinity and functional activity of antibodies.

Figure 1

A. ELISA of the hGCGR-expressing cell line with candidate antibodies including NPB112 (designated as Ab8). B. Cell-based cAMP assay using hGCGR-expressing cell lines with candidate antibodies including NPB112 (designated as Ab8). C. Cell-based cAMP assay with NPB112 using recombinant cell lines expressing different GCGRs from human, murine, and cynomolgus monkey. D. Plasma concentration-time profiles of NPB112 in male Sprague-Dawley rats following a single intravenous or intraperitoneal administration of 5 mg/kg NPB112. Data are expressed as mean ± S.D. (n = 5/time point/group).