Purification of tobacco INH from a salt-eluted
cell wall protein fraction from transformed tobacco cells. Lane M,
Marker proteins; lane 1, ammonium sulfate fraction (40–85%
saturation); lanes 2 and 3, CWI activity peak fractions from
cation-exchange chromatography, pH gradient (lane 2) and NaCl gradient
(lane 3; Weil and Rausch, 1994); lane 4, electroeluted INH protein from
the NaCl-gradient peak fraction (see lane 3); lane 5, immunoblot of
ammonium sulfate fraction (see lane 1) with affinity-purified
polyclonal antiserum directed against INH.