Figure 4.

Protein sequence alignment of Nt-inh1 with putative homologs from A. thaliana (A.t.) and C. unshiu (C.u.). The A. thaliana cDNA clone At-inhh (A.t.; EMBL nucleotide sequence database, accession no. Y12807) is the full-length clone corresponding to the A. thaliana EST clone with the accession no. T88450. The partial C. unshiu cDNA sequence (C.u.) represents an EST clone with the accession no. C22245. The putative signal sequences are underlined, and the most likely predicted cleavage sites are marked by upward arrows. Note that the putative signal peptide of the C. unshiu EST clone shows a very low score (Von Heijne, 1986). Conserved Cys residues are indicated by downward arrows. Amino acid residues identical between at least two sequences are in bold type. Except for the N-terminal sequences (upstream from Asn-20 in Nt-inh1), the predicted mature protein sequences were aligned using the CLUSTAL program from PC Gene (Intelligenetics, Mountain View, CA).