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Transcript amounts for CWI and Nt-inh1 in different tissues of tobacco plants as determined by RNA-blot analysis. Total RNA was isolated from different tissues of 7- and 15-week-old tobacco (cv SNN) plants. For 15-week-old plants leaves were numbered starting from the oldest (senescent) leaf. For each tissue 10 μg of total RNA was loaded per lane. Blots were hybridized with coding region probes for CWI (Krausgrill et al., 1996) and Nt-inh1.
