Figure 2. Specificity of HPV type-specific antibodies against different HPV E1?E4 proteins by ELISA and Western blotting.

Optical density measurements from ELISA on a panel of 10 recombinant maltose-binding E4 proteins (HPV-16, 18, 31, 33, 35, 39, 45, 52, 58, and 59) used to evaluate the specificity of on M16E4_35–42, R18E4_53–60 and R58E4_23–30 polyclonal antibodies (A) and MoAb16E4_35–42 monoclonal antibody (B). Cross-reactive TVG405 was used for comparison (C) and the relative abundance of the various MBP proteins is shown following staining with Coomassie blue (lower panel of C). Western blot results are shown as inserts under the corresponding graphs presenting the ELISA results.