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. 2012 Dec 3;7(12):e49707. doi: 10.1371/journal.pone.0049707

Figure 7. iKras*p53* cells reactivate Kras* expression independently of doxycycline regulation.

Figure 7

(A) Tumor volume measured over time for iKras*p53*-1 cells transplanted subcutaneously in NOD/SCID mice. The yellow line indicates the presence of doxy, black lines indicate the absence of doxy, arrows indicate harvest time-points. N = 5. (B) Histology and phospho-ERK1/2 expression (inset) of iKras*p53*-1 tumors harvested during the initial growth phase and of relapsed tumors. Scale bar 100 um. (C) Tumor formation, regression, and relapse in NOD/SCID mice injected subcutaneously with primary cell line iKras*p53*-2. N = 5. A second cohort of NOD/SCID mice were injected with iKras*p53*-2 cells, but maintained in the absence of doxy which do not develop tumors. N = 10. The yellow line indicates the presence of doxy, black lines indicate the absence of doxy, arrows indicate harvest time-points. (D) Histology and pERK1/2 (inset), Ki67 and SMA expression of iKras*p53*-2 tumors during growth, regression, and relapse phases. Scale bar 100 um. (E) Quantitative PCR for oncogenic Kras* and myc expression in iKras*p53*-2 tumors.