Expression of ICAM-1 by primary oral epithelial cells (NHOK) and six oral epithelial cell lines (HOK-18A, 1483, HEp-2, SCC-4, SCC-9, and Tu-177). Flow cytometric analysis of ICAM-1 expression on (A) NHOK and three oral cell lines containing HPV-18 and (B) three HPV-negative oral cancer cell lines. Shaded areas represent constitutive ICAM-1 expression and non-shaded areas depict staining with an isotype control antibody. (C) Northern blot analysis of ICAM-1 mRNA expression in oral epithelial cells. (D) Relative signal densities of ICAM-1 mRNA among the cells measured by NIH imaging software. Data are from a representative experiment.