Table 2.

Chemicals necessary for the preselective and selective amplification.

Chemicals	μl per preselective sample	μl per selective sample
ddH2O	5.86	5.50
10x Red Taq Buffer	1.14	1
Primers	0.58 of the mixed primer pair	0.54 MseI primer (5 μM) 0.54 EcoRI primer (1 μM)
dNTPs (10 mM)	0.22	0.22
Red Taq (1U/ μl)	0.2	0.2