. 2012 Aug 21;13(4):1103–1109. doi: 10.1208/s12249-012-9830-3

Table III.

Chromatography Conditions

Parameter	Genistein	Probucol	Nifedipine	Indomethacin
Column	Alltech, Alltima HP C18 column, 5 μm (150 × 4.6 mm)	Phenomenex®, Kinetex® C18 column, 2.6 μm (150 × 4.6 mm)	Phenomenex®, Luna® Phenyl-Hexyl, 3 μm (150 × 4.6 mm)	YMC America, YMC-Pack, ODS-A, 5 um (150 × 4.6 mm)
Column temperature (°C)	25	25	25	25
Mobile phase	A: 0.1% Acetic Acid/Water	A: Water	A: Water	A: 0.1% TFA/Water
Mobile phase	B: ACN	B: Methanol	B: ACN	B: 0.1% TFA/ACN
Gradient	Gradient	Isocratic 95% B for 11 min	Gradient	Isocratic 60% B for 10 min then 95% B rinse for 5 min
Gradient	5–40% B for 12 min then 40% B for 4 min and 95% B rinse for 8 min	Isocratic 95% B for 11 min	65–95% B for 10 min then 95% B rinse for 5 min	Isocratic 60% B for 10 min then 95% B rinse for 5 min
Injection volume (μL)	5	5	5	5
Wavelength (nm)	259	242	236	240
Run time (min)	30	11	20	20