Figure 3. HEDS decreases levels of protein thiols (PSH) in glucose-deprived human colon cancer cells independent of p53 status.
The effect of glucose deprivation on intracellular protein thiol levels was determined in the absence or presence of HEDS at the indicated concentration in cells that are radiation-sensitive and p53 wild-type HCT116 (A) or radiation-resistant and p53 mutant HT29 (B). Mean for three to five independent experiments are shown with SD. A statistically significant reduction in PSH levels in cells cultured in glucose-deprived media occurred after HEDS treatment (0.7 mM, *P<0.01; 3.3 mM, **P<0.001).