Fig. 2.

GPER, ESR1, ESR2, and GPR30 expression and effect of G1, estradiol, and CTx on EMMPRIN level in conditioned medium. A, Expression of GPER, ESR1, and ESR2, which encode GPR30, ERα, and ERβ, respectively, reported by RT-PCR of EEC mRNA with gene-specific primers. B, Immunoblot detection of GPR30 in EEC lysate. C, Immunoblot detection of EMMPRIN in concentrated conditioned medium after treatment with 0.1% DMSO (DM), 20 nm G1, 0.1% ethanol (Et), 20 nm estradiol (E), 10 μm CTx, or 200 nm G15 alone (G15) or supplemented with either 20 nm G1 (G1+G15) or 20 nm estradiol (G15+E) for 48 h.