Fig. 4.
PTEN immunohistochemistry of germline PTENmut+-related thyroid cancer showing both nuclear and cytoplasmic loss of PTEN distinct from that seen in PTENwt/vus-related thyroid cancer (×40 magnification). A, Papillary thyroid cancer with absent (−) PTEN staining; underlying c.210–1G→A germline PTEN mutation with blood PTENQ1 expression; B, follicular variant of papillary thyroid cancer with weak (+) nuclear PTEN staining; underlying c.80-?_164+?del germline PTEN mutation with blood PTENQ1 expression; C, papillary thyroid cancer with strong (3+) cytoplasmic PTEN staining; PTEN wild-type with blood-PTENQ3 expression; D, papillary thyroid cancer with moderate (2+) nuclear PTEN staining; PTEN wild-type with blood-PTENQ1 expression.