Figure 5. Bridge B2a during subunit rotation.

Rotation of the small subunit during translation leads to a lateral displacement of h44 in the small subunit by 6° towards the E site. Upon rotation H69 in the large subunit compresses by 5° in order to maintain its inter-subunit contacts with h44. Nucleotide A1928 is nearly invariant in position and is shown for reference. Ribosomal RNA of the fully rotated ribosome is shown in blue (30S) and gray (50S) whereas rRNA of the unrotated ribosome is shown in red. Inset, icon of the ribosome indicating the direction of view. Reproduced with permission from [26].