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. Author manuscript; available in PMC: 2013 Nov 30.
Published in final edited form as: Mol Cell. 2012 Oct 11;48(4):655–661. doi: 10.1016/j.molcel.2012.09.003

Figure 1. Purification and thermodynamic analysis of the assembly of Leptin/LEP-R complexes.

Figure 1

A) Isothermal titration calorimetry for the assembly of Leptin and LEP-R. The inset table lists the thermodynamic parameters for the binding isotherm. B) Size exclusion chromatography profile and SDS-PAGE analysis of purified liganded LEP-R[D1-D7].