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. Author manuscript; available in PMC: 2013 Oct 22.
Published in final edited form as: Matrix Biol. 2012 Oct 22;31(7-8):380–388. doi: 10.1016/j.matbio.2012.10.001

Fig. 2.

Fig. 2

SDS/PAGE analysis of wild-type and variants of MMP-2. (A) The purity of recombinant proteins was confirmed by 10% SDS/PAGE under reducing conditions. Bands were visualized by Coomassie Blue staining. (B) In order to verify the proteins’ structural integrity, migration of wild-type and MMP-2 variants was compared by SDS/PAGE using 10% gels in the presence (+) or absence (−) of 100 mM dithiothrietol (DTT) followed by silver staining.