Fig. 3.

CD spectral analysis of wild-type and variant MMP-2 enzymes. CD studies were performed on a Jasco J-810 spectropolarimeter using a 1 mm rectangular cell. Protein samples were dissolved at 0.25 mg/ml in 50 mM Tris-HCl (pH 7.4). Wavelength scans were performed at 20 °C from 195 to 250 nm at scan rate of 50 nm/min. For each sample three scans were averaged and corrected for background absorbance.