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. Author manuscript; available in PMC: 2013 Oct 22.
Published in final edited form as: Matrix Biol. 2012 Oct 22;31(7-8):380–388. doi: 10.1016/j.matbio.2012.10.001

Fig. 5.

Fig. 5

Degradation of denatured type I collagen by wild-type and MMP-2 variants. (A)Effects of alanine substitutions on the enzymatic activity of MMP-2 were analyzed by zymography using 10% minislab gels co-polymerized with gelatin. Gels were incubated 4 h at 37 °C with collagenase assay buffer and stained with Coomassie Blue. (B) Gels were scanned and band intensities were quantified using Kodak 1D imaging software. Data are expressed in per cent of activity of wild-type MMP-2. Bars represent means with S.D. from three separate experiments.