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. Author manuscript; available in PMC: 2013 Nov 30.
Published in final edited form as: Mol Cell. 2012 Oct 18;48(4):532–546. doi: 10.1016/j.molcel.2012.09.011

Figure 4. Tf clustering at centromeres is compromised in condensin mutant.

Figure 4

(A) The condensin mutation, cut14-208, impairs Tf clustering. FISH analysis was performed as described in Figure 2A. Black bar indicates 1 μm.

(B) Centromeric localization of Tf cluster is compromised in condensin mutant cells. The experiment was carried out as described in Figures 3D and 3E.

(C) Co-IP analysis investigating an interaction between Pku80-Flag and Cut3-Myc.

(D) ChIP result showing enrichment of the condensin subunit Cut14-Pk at Tf elements in wt and pkuΔ cells.

(E) ChIP result showing enrichment of Pku70-Pk and Pku80-Pk at Tf1-10 in wt and cut14-208 condensin mutant cells.

Data are represented as mean +/− SD.

n.s. indicates P > 0.05.