(A) The condensin mutation, cut14-208, impairs Tf clustering. FISH analysis was performed as described in Figure 2A. Black bar indicates 1 μm.
(B) Centromeric localization of Tf cluster is compromised in condensin mutant cells. The experiment was carried out as described in Figures 3D and 3E.
(C) Co-IP analysis investigating an interaction between Pku80-Flag and Cut3-Myc.
(D) ChIP result showing enrichment of the condensin subunit Cut14-Pk at Tf elements in wt and pkuΔ cells.
(E) ChIP result showing enrichment of Pku70-Pk and Pku80-Pk at Tf1-10 in wt and cut14-208 condensin mutant cells.
Data are represented as mean +/− SD.
n.s. indicates P > 0.05.