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The effect of microinjection of syntide-2 on GA-induced cytoplasmic Ca2+ elevation. Freshly isolated protoplasts were microinjected with dextran-conjugated indo-1, with or without 50 μm syntide-2. These protoplasts were then treated without hormone (control) or with 5 μm GA and their cytoplasmic [Ca2+] was monitored at the indicated times using confocal ratio imaging. The results represent the mean ± se of at least nine microinjected protoplasts per treatment.
